Compulsory subject, 6 credits
Hours/week: lecture-2, practical works-3
Structure by years of study. The analytical programme is addressed to students of the first year of the Faculty of Medicine
General objectives: The objective of Cellular and Molecular Biology is to provide first-year students with the informational and logistical support necessary to acquire knowledge of general cell concepts, the molecular basis of cell organization (structure and ultrastructure of the eukaryotic cell) and physiological and pathological cellular mechanisms.
Specific objectives: At the end of the training process in this discipline the student will be familiar with modern notions and techniques of cell and molecular biology: light and electron microscopy, cell culture, cell fractionation methods, spectrophotometry, DNA-RNA technology (PCR, sequencing, Real-Time PCR), and will be able to integrate the knowledge of Cell and Molecular Biology with the notions acquired in the other disciplines.
Related disciplines: Biochemistry, Genetics, Microbiology, Histology, Physiology, Physiopathology, Morphopathology.
Evaluation mode: Written exam (course) - 60%, practical - 20%, tests during the semester - 20%.
Course content: Origin and evolution of eukaryotic cells; Molecular components of the cell; Molecular architecture of the cell membrane; Transport function of the cell membrane; Cell signalling; Cell cytoskeleton; Extracellular matrix; Cell nucleus; Intracellular trafficking of macromolecules; Energy conversion. Mitochondria; Cancer cell; Nerve cell.
Content of practical work: Cell Biology Module: Principles of optical microscopy; Electron microscope; Techniques for preparing tissues for light and electron microscopic examination; Cell cultures; Cell fractionation methods; Molecular Biology Module: DNA and RNA isolation and purification, DNA/RNA amplification - PCR technique; Nucleic acid quantification methods. Electrophoresis - types of electrophoresis; Mutation detection techniques; Molecular methods used for non-self DNA and RNA detection.
BIBLIOGRAPHY:
1. Molecular Biology of the Cell Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan,
Martin Raff, Keith Roberts, and Peter Walter. Garland Science; 2015.
2. http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mboc4.TOC&depth=2
3. Course taught
4. Lucrări practice de Biologie Celulară și Moleculară, Autori: Mihai Ioana, Ioana Streață, Răzvan
Mihail Pleșea, Anca Lelia Riza, Bogdan Petre Stănoiu, Editura Medicală Universitară Craiova 2022
5. Link: Teaching Materials - Discipline Cell and Molecular Biology:
6. Biologie Celulară și Moleculară. Ediție adăugită și revizuită – Sub redacția Prof. univ. dr. Sin A.;
Autori: Tilinca M., Marian R., Vartolomei M.D., Zazgyva A., Iacob O.; University Press 2015
7. Steven R. Goodman , Goodman’s Medical Cell Biology, Fourth Edition, Academic Press, 2020
8. Manuela Curticapean, Tehnici de biologie moleculară și genetică, University Press, 2016